The Phosphosite Library
To identify phosphorylation sites that might regulate AlphaFold-Multimer's protein-protein interaction (PPI) predictions, we mapped experimentally determined phosphosites onto predicted interfaces. We then constructed a set of phosphosites which we predict stimulate PPIs with our Classifier of Phosphosite Interactions (COPI), which uses spatial and chemical features from the phosphosite’s local structure prediction. In addition, we applied simple screening criteria to find phosphosites which might inhibit our PPI predictions. The table below of predicted phospho-regulated interactions can be sorted by diverse features of each protein pair. Clicking on a pair retrieves an interactive structure viewer and information about the putative regulatory kinase. Our data consist of phosphosites from nearly 40,000 unique PPI predictions which include proteins across the human proteome. Please find relevant sites by searching for your protein of interest.
Filters
| Chain A / Chain B i |
phos_chain i |
phos_res i |
contact i |
SPOC i |
models i |
avg_PAE i |
avg_pLDDT i |
ht-lt_refs i |
Avg. COPI i |
kinase i |
source i |
|---|