The Human Predictome
Human protein-protein interactions predicted with Alphafold-Multimer ( video tutoriali )
WARNING: In silico screening has many limitations; all predictions require experimental validation.
Protein-protein interactions (PPIs) underlie virtually all biological processes. To screen for human PPIs proteome-wide, we developed a two-step in silico pipeline based on AlphaFold-Multimer. This pipeline identified ~16,000 high confidence PPIs (~90% precision) and ~112,000 lower confidence PPIs (~50% precision). We have also performed more focused in silico PPI screens.
Coupled with experimental validation, in silico PPI screening has led to new mechanistic insights in DNA replication, DNA repair, ubiquitin signaling, and other pathways. The human predictome is expected to greatly advance our understanding of the PPIs that underlie cell physiology, laying the foundation for the development of new drugs to fight human disease.